Fig. 2
From: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Identification and knockout of non-essential secreted proteins. A Schematic of CRISPR-Cas9 knockout screen to determine essentiality. B Gene set enrichment analysis of genes in the K. phaffii secretome weighted by their essentiality score. C Plot of gene essentiality and relative abundance of proteins in production phase culture supernatant. Genes with an essentiality score of > 0.5 were considered likely to be essential. D Knockout efficiencies of sequential disruption of non-essential genes in the K. phaffii secretome. Knockout genotypes were determined by Sanger sequencing of the targeted locus for up to 16 colonies per transformation