Fig. 4

Putative mechanism of competence formation and BsuMI regulation during the Spizizen transformation Combining the analysis results about the differential genes in the three strains, we proposed the metabolic flow and the regulatory role of the BsuMI methylation subunit during the competence formation process. A the heatmap of different genes enriched in the starch and sucrose metabolism in three strains under the two cultivation conditions. B the heatmap of different genes enriched in the phosphotransferase system in three strains under the two cultivation conditions. C plasmids transformations in constructed strains with silenced manP expression. All experiments were conducted with three biological replicates. p < 0.05. D mechanism of competence formation and BsuMI regulation during the Spizizen transformation