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Table 2 5’ UTR sequences for expression of Fru regulon

From: Synthetic redesign of Escherichia coli W for faster metabolism of sugarcane molasses

Strain

5’ UTR sequence (5’-3’)*

Predicted expression level (a.u.)

F1

CTTTTTCACTATTTATTCACACAT

3,231

F2

TCAAATAAAATCTAAGAAAACAAGAATC

12,136

F3

CAGACTATCTATCTATAAGGTTTTTTT

62,626

F4

AGCTACAAAGCCAACAACAATAAGGAGTTTTTTA

1,386,568

  1. *Underlined letters indicate the ribosome binding site