Fig. 4
Screening of selected::PhrtB-pfkA and::PhrtB-aceE clones for increased hrtB expression. After a pre-screening in unicates (Figure S3 and S4), several clones of C. glutamicum (A)::PhrtB-pfkA and (B)::PhrtB-aceE were selected and inoculated together with the WT in triplicates to an OD600 of 1 in CGXII media containing 2% glucose and 100 µM FeSO4. Orange bars represent clones selected for whole genome sequencing. Specific fluorescence at a time point of 4 h (highest hrtB reporter output [20]) is given by measured fluorescence (a.u.) divided by respective backscatter (a.u.) measured in a microbioreactor system. n = 3 biological replicates. fALE = clones of the fluorescent ALE