Table 1 Plasmids and strains used in this study
Names | Relevant characteristics | Sources |
|---|---|---|
pETDuet-1 | ColEl origin, AmpR | Lab collection |
pCDFDuet-1 | CloDF13 origin, SmR | Lab collection |
pACYCDuet-1 | P15A origin, CmR | Lab collection |
pCDTI | T7 EcThiM, MthIPK, pCDFDuet-1 ori, SmR | This study |
pACEIG | T7 ScERG20(F96C), ScIDI, TcGGPPS, pACYCDuet-1 ori, CmR | This study |
pETDN | T7 NaGLS, pETDuet-1 ori, AmpR | This study |
pETDNT | T7 NtGLS, pETDuet-1 ori, AmpR | This study |
pETDNTO | T7 NtoGLS, pETDuet-1 ori, AmpR | This study |
pETDL | T7 LfGLS, pETDuet-1 ori, AmpR | This study |
pETDS | T7 SdGLS, pETDuet-1 ori, AmpR | This study |
pETDC | T7 CaGLS, pETDuet-1 ori, AmpR | This study |
E. coli TOP10 | F–mcrAΔ(mrr-hsdRMS-mcrBC), Φ80, lacZΔM15 | Lab collection |
E. coli LSC05 | E. coli BL21(DE3), ΔaphA, ΔyqaB, ΔphoA | Lab collection |
E. coli TI | E. coli LSC05/pCDTI | This study |
E. coli EIG | E. coli LSC05/pACEIG | This study |
E. coli DN | E. coli LSC05/pETDN | This study |
E. coli GP | E. coli LSC05/pCDTI + pACEIG | This study |
E. coli GL01 | E. coli LSC05/pCDTI + pACEIG + pETDN | This study |
E. coli GL02 | E. coli LSC05/pCDTI + pACEIG + pETDNT | This study |
E. coli GL03 | E. coli LSC05/pCDTI + pACEIG + pETDNTO | This study |
E. coli GL04 | E. coli LSC05/pCDTI + pACEIG + pETDL | This study |
E. coli GL05 | E. coli LSC05/pCDTI + pACEIG + pETDS | This study |
E. coli GL06 | E. coli LSC05/pCDTI + pACEIG + pETDC | This study |