Fig. 3

Identification of sphingolipids production in S1P-producing strains by flask fed-batch fermentation. Both the DDLAOgS (A) and DDLAOsS (B) strains were cultivated in YPD medium containing 60 g/L glucose under aerobic conditions with high initial cell density. After depletion of initial glucose, galactose or sucrose was feed for inducing sphingosine kinase, respectively. Cell growth and sphingolipids levels were measured during 60 h of cultivation. Error bas indicates standard deviations of three independent experiments