Fig. 3
From: Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha
Optimization of promoter sequences for crRNA array. A Schematic illustration of the construction of a crRNA expression cassette with Type III promoters and PolyT terminator containing different lengths of 5' UTR regions of unknown function. B Editing efficiency when transcribing crRNA using different Type III promoters (5 s rRNA and tRNA). C Effect of using tRNAAGA with different lengths of upstream regions as a promoter on editing efficiency during crRNA transcription. The data are represented as mean ± SD. Data are presented as means of three biologically independent samples. Black asterisks indicate statistical significance as determined using paired t test (**p < 0.01; ***p < 0.001; ns.: no significant difference)