Fig. 4

CRISPR-Cpf1 system efficiency in editing multiple genes with a crRNA array. A Schematic representation of the construction of the crRNA array expression cassette used for multi-gene editing. B Editing efficiency of CRISPR-Cpf1 on single and dual gene ADE2-HIS4. C Editing efficiency of CRISPR-Cpf1 on single and double gene ADE2-GUT1. D Editing efficiency of CRISPR-Cpf1 on the dual gene HIS4-GUT1. E Editing efficiency of CRISPR-Cpf1 on single, double, and the triple gene ADE2-HIS4-GUT1. The data are represented as mean ± SD. Data are presented as means of three biologically independent samples. Black asterisks indicate statistical significance as determined using paired t test (ns.: no significant difference)