Fig. 6

Improving the efficiency of multi-gene integration and large DNA fragment deletion based on homologous recombination. A Schematic representation of the one-step integration of three genes ADE2, HIS4, and LEU2. B Diagram illustrating deletions of different large DNA fragment lengths (10 k, 15 k, and 20 k). C The efficiency of multi-gene integration. The efficiency of one-step integration of three genes was evaluated by comparing the growth status of transformants on MD plates and the MD plates containing leucine, adenine, and histidine after co-transformation of a plasmid containing a crRNAs array and donor DNA harboring the ADE2, HIS4, and LEU2 expression cassettes (n = 180). D Efficiency of CRISPR-Cpf1-mediated deletion of large genomic fragments of varying lengths. The data are represented as mean ± SD. Statistical analysis using paired t test showed no significant difference (ns.) in deletion efficiency between 10 K, 15 K, and 20 K