Fig. 1

SDS-PAGE analysis of rhBiP secretion into different growth media in P. pastoris. Lanes were loaded with samples of rhBiP-producing clone ppBiP10 cultivated in the shake flasks with indicated culture media: YEPM– complex media; BSM– basal salt medium; BSM + YE + PE– basal salt media supplemented with yeast extract and soy peptone; BSM + YE– basal salt media supplemented with yeast extract; BSM + PE– basal salt media supplemented with soy peptone. C lane– YEPM culture media from P. pastoris transformed with control pPIC3.5 K vector. All culture growth media were concentrated 10x and 7.5 µl (obtained from 75 µl of culture medium) was loaded onto the lane. M lane – unstained protein ladder (ThermoScientific, cat. no. 26614)