Fig. 2

Evaluation of host strain modifications for protein display efficiency. (a) Flow cytometry analysis of Saccharomyces boulardii (Sb) transformed with a plasmid expressing A2. (b) Flow cytometry analysis of Sb transformed with a plasmid expressing S1. Mean fluorescence intensity (MFI) values were measured for yeast-enhanced GFP (yeGFP) and Alexa 594 signals to assess protein display efficiency. Error bars indicate standard deviation (n = 3). Statistical significance was evaluated using one-way ANOVA (p < 0.05), with different letters denoting significant differences between groups. Host strain modifications are labeled below the x-axis as follows: ↑ indicates overexpression, and Δ indicates deletion of the targeted gene