Fig. 5

Radial colony growth and antifungal activity of T. atroviride wild type in comparison to its ∆pks1 mutants. (A) Colony diameter measurements of T. atroviride wild type (WT; blue) and pks1 deletion mutants ∆pks1A, ∆pks1B, ∆pks1C (purple tones) on PDA at 25 °C under light-dark conditions. No statistically significant differences between the WT and the ∆pks1 mutants were found. A representative image out of three biological replicates (n = 3) is shown on the right. (B) Inhibition assay to assess the antifungal activity of metabolites secreted by T. atroviride WT and ∆pks1A against B. cinerea (Bc; top row) and R. solani (Rs; bottom row) on PDA under reduced light conditions. Purple lines indicate the area originally covered by the T. atroviride colony after 2 days of growth before the test fungus was inoculated. Representative images out of four biological replicates (n = 4) are shown. Inhibition index represents the inhibitory activity of T. atroviride WT and ∆pks1A against the respective fungal test organism. Results shown are means ± standard deviation. The asterisks indicate statistically significant differences of the ∆pks1A mutant compared to the WT