Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device

Table 1 Sequence information for UP and spacer

Region	Sequence (5′-3′)	Source^*
UP1	GGATCACTAGAAAATTTTTTAAAAAATCTC	[40]
UP2	GAGATTTTTTAAAAAATTTTCTAGTGATCC	[40]
UP3	AAAAATTTTTTAGCAAAGC	[38]
Spacer1	aaaagcgcgcgattatg	P₄₃
Spacer2	CAGAAAAAATCGTG	P_spoVG
Spacer3	caagaaaatgTATGT	AD
Spacer4	AAAACATTTTTTTCATT	P₁₀
Spacer5	aaagaaaattttttg	AD
Spacer6	caaTaaaatTTtttg	AD

^*AD indicates that the corresponding sequence was artificially designed in this study

ISSN: 1475-2859