Fig. 9

Schematic diagram of the dimerization of rMaSp1s. (a) The rMaSp1s C-terminal structural domain. (b) SDS-PAGE analysis of rMaSp1s before and after dimerization. Protein samples were prepared by mixing with sample buffer containing thiol-reducing agent, followed by heating at 100 °C for 5 minutes in a boiling water bath. The gel shows significant amounts of both dimer and monomer forms of rMaSp1s. The presence of reducing agents and heating may influence the observed band patterns